Dynamic assessment of hemostasis in canines: establiDynamic assessment of hemostasis in caninesues with rotational thromboelastometry
Establishing normal values with rotational thromboelastometry
DOI:
https://doi.org/10.29155/VET.62.225.8Keywords:
Rotem, Hyperfibrinolysis, Hemostasis, Coagulation, DogsAbstract
Coagulation disorders represent a common diagnostic challenge in veterinary medicine. The objective of this study was to establish reference intervals for EXTEM and INTEM test parameters using ROTEM® delta in healthy canines, in order to facilitate the clinical interpretation of viscoelastic tests in this species. Thirty clinically healthy dogs, older than one year, were studied and underwent ROTEM® delta testing in the EXTEM and INTEM channels, analyzing parameters such as Clotting time (CT), Clot formation time (CFT), alpha angle, Amplitude at 10 minutes (A10), Amplitude at 20 minutes (A20), Clot firmness (CFF), and Lysis at 30 minutes (LI30). The reference intervals obtained were consistent between animals and comparable to those reported internationally, with some variations attributable to regional factors. This study provides the first reference values in Argentina for ROTEM® delta in canines, constituting a useful tool for the diagnosis and management of hemostatic disorders in the clinical setting.
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Birkbeck, R., Humm, K., & Cortellini, S. (2019). A review of hyperfibrinolysis in cats and dogs. Journal of Small Animal Practice, 60(11), 641-655. https://doi.org/10.1111/jsap.13068
Buriko, Y., & Silverstein, D. C. (2018). Establishment of normal reference intervals in dogs using a viscoelastic coagulation monitor (VCM) and validation of the VCM device using thromboelastography (TEG) [Abstract]. Journal of Veterinary Emergency and Critical Care, 28(S1), S27. https://doi.org/10.1111/vec.12758
Burton, A. G., & Jandrey, K. E. (2020). Use of thromboelastography in clinical practice. Veterinary Clinics of North America: Small Animal Practice, 50(6), 1397-1409. https://doi.org/10.1016/j.cvsm.2020.08.001
Clinical and Laboratory Standards Institute. (2010). Defining, establishing, and verifying reference intervals in the clinical laboratory; Approved guideline (3rd ed., EP28-A3c). CLSI.
Geffré, A., Concordet, D., Braun, J. P., & Trumel, C. (2011). Reference Value Advisor: A new freeware set of macroinstructions to calculate reference intervals with Microsoft Excel. Veterinary Clinical Pathology, 40(1), 107-112. https://doi.org/10.1111/j.1939-165X.2011.00287.x
Goggs, R., Brainard, B., De Laforcade, A. M., Flatland, B., Hanel, R., McMichael, M., & Wiinberg, B. (2014). Partnership on Rotational ViscoElastic Test Standardization (PROVETS): evidence‐based guidelines on rotational viscoelastic assays in veterinary medicine. Journal of Veterinary Emergency and Critical Care, 24(1), 1-22. https://doi.org/10.1111/vec.12144
Martin, A., Kutter, A., & Sigrist, N. (2020). Rotational thromboelastometry (ROTEM) parameters in dogs with haemoperitoneum and their associations with clinical and laboratory signs. Schweizer Archiv fur Tierheilkunde, 162(3), 153-161. https://doi.org/10.17236/sat00249
Ozarda, Y. (2016). Reference intervals: Current status, recent developments and future considerations. Biochemia Medica, 26(1), 5-17. https://doi.org/10.11613/BM.2016.001
Pereira, J. M., Rohn, K., & Mischke, R. (2020). Reference intervals for rotational thromboelastometry measurements using the ROTEM® delta device in dogs. Research in Veterinary Science, 130, 26-32. https://doi.org/10.1016/j.rvsc.2020.01.019
Sigrist, N.E., Jud Schefer, R.J., & Kutter, A.P.N. (2018). Characteristics of hyperfibrinolysis in dogs and cats demonstrated by rotationa thromboelastometry (ROTEM), The Veterinary Journal, 242, 67-73. https://doi.org/10.1016/j.tvjl.2018.11.002
Sigrist, N. E., Hofer-Inteeworn, N., Jud Schefer, R., Kuemmerle-Fraune, C., Schnyder, M., & Kutter, A. P. (2017). Hyperfibrinolysis and hypofibrinogenemia diagnosed with rotational thromboelastometry in dogs naturally infected with Angiostrongylus vasorum, Journal of Veterinary Internal Medicine, 31(4), 1091-1099. https://doi.org/10.1111/jvim.14723
Thawley, V. J., Sánchez, M. D., Drobatz, K. J., & King, L. G. (2016). Retrospective comparison of thromboelastography results to postmortem evidence of thrombosis in critically ill dogs: 39 cases (2005–2010). Journal of Veterinary Emergency and Critical Care, 26(3), 428-436. https://doi.org/10.1111/vec.12441
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